Mfsd2a Promotes the Proliferation, Migration, Differentiation and Adipogenesis of Chicken Intramuscular Preadipocytes

Intramuscular fat (IMF) content is a crucial parameter for estimating meat quality. Growing evidence indicates that gene regulation plays an important role in IMF deposition. This study aimed to determine the function of Mfsd2a chicken intramuscular preadipocytes. In present study, high mRNA levels were observed liver and adipose tissues broilers. Subsequently, we synthesized small interfering RNAs silence expression The following results suggested CDK2, PCNA, CCND1, CCND2 MKI67 inhibited, with CCK-8 EdU assays revealing cell proliferation was inhibited. Scratch test showed migration ratios declined. We also found silencing decreased PPARγ, RXRG their target genes. similar some key genes contribute lipid synthesis, including C/EBPα, C/EBPβ, FABP4, FASN, ACACA ACSL1. Finally, Oil red O staining accumulation blocked after silencing. conclusion, our implied promotes preadipocytes, as well differentiation adipogenesis through PPARγ signaling pathway, which may provide potential improve